It is growing in nepal, srilanka, bacopa monnieri is an annual creeping herbal plant, in wet and marshy regions of the eastern mediterranean which belongs to family. Induction of callus were carried out in success and the callus were multiplied using the bubble column type of bioreactor. Bacopa monnieri and the benefits to brain health by. The present study aimed to standardize an improved method for micropropagation and in vitro biomass production of bacopa monnieri. Bacopa monnieri is a highly valuable medicinal plant from the scrophulariaceae family has been proven its.
Pdf effect of growth hormone on shoot bud multiplication of. Tissue culture tissue culture techniques are very widely used and its most important application is the micro propagation. Comparative phytochemical evaluation of natural and. The pharmacological properties of bacopa monnieri were studied extensively and the activities were attributed mainly due to the presence of characteristics saponins called as bacosides 8. Apr 12, 2019 heavy metals are released into the environment as a result of anthropogenic activities such as melting metallic ores, industrial productions, power stations, and pesticides. Micropropagation of bacopa monnieri and enhancing bacoside a. Bacopa monnieri article pdf available in medicinal plants international journal of phytomedicines and related industries 112. Plant tissue culture remains one of the most basic biotechnological techniques with its varied and vast applications.
Bacopa monnieri and ldeprenyl differentially enhance the activities of antioxidant enzymes and the expression of tyrosine hydroxylase and nerve growth factor via erk 12 and nfjb pathways in the spleen of female wistar rats. Second aim is to evaluate the antimicrobial potency of in vitro cultured and. Bacoside a is one of the active principles in the plant, responsible for improving memory. Though effects of this nature are usually studied in the elderly, bacopa monnieri appears to affect young people as well, making it a useful nootropic. Owing to the resurgence in herbal based therapies, plants which serve as raw materials are required in large quantities to meet their increasing demand. A number of hybrids of commercial importance are routinely propagated by tissue culture methods.
Heavy metals are released into the environment as a result of anthropogenic activities such as melting metallic ores, industrial productions, power stations, and pesticides. In vitro rapid and mass multiplication of highly valuable. Tissue culture of endangered brahmi bacopa monnieri. Plant tissue culture vessels with their caps or closures make the boundaries. An efficient micropropagation protocol of bacopa monnieri l. A threemonth trial conducted by australian scientists suggests that it may support learning, moderate stress, and promote memory. Family scrophulariaceae, genus bacopa and species monnieri. In india and the tropics it grows naturally in wet soil, shallow water and marches of bacopa monnieri brahmi. Pdf on jan 1, 2019, bharti chaudhry and others published micropropagation of a medicinally important plant.
Additional efforts are employed through various conventional and nonconventional ways. Using cyanobacterial mediaa novel approach and effect of phytoregulators on in vitro micropropagation. So, the aim of this investigation so, the aim of this investigation is to establish an efficient procedure for micropropagation and plant preservation via synthetic seeds, estimate. Original contribution open access improved micropropagation. Clonal propagation through tissue culture popularly called micropropagation. Pdf in vitro micropropagation of brahmi bacopa monniera l. Micropropagation of endangered medicinal plant bacopa. C ognitive enhancement typically exacts a toxicological and psychological toll. Bacopa monnieri can also be supplemented in a leaf or powder form. Micropropagation, conservation, phytochemicals and genetic. Micropropagation of bacopa monnieri through protoplast.
Pdf effect of growth hormone on shoot bud multiplication. While the demand for medicinal plants is growing, some of them are increasingly being threatened in their natural habitat. May 18, 2018 bacopa monnieri has been shown to increase cerebral blood flow independent of overall blood pressure. Micropropagation of bacopa monnieri through protoplast t. Evaluation of micropropagation system of bacopa monnieri l. A protocol has been developed for micropropagation of bacopa monnieri l wettst, a medicinal plant of high commercial potential with legendary reputation as a memory vitalizer. Bacopa contains contains anthraquinones, phytosterol, and terpenoids, as well as numerous biologically active compounds, such as alkaloids brahmine and herpestine. An improved protocol for in vitro multiplication of bacopa. Effect of growth hormone on shoot bud multiplication of bacopa monnieri.
Chlorophyll estimation the chlorophyll content chl a and chl b of fresh leaves of bacopa monnieri was extracted using 80% acetone and absorptions were read on a. Cost effective protocol for micropropagation of bacopa monnieri. Medicinal plants may be considered as new sources for producing antibacterial agents. An animal study suggests the potential for bacopa to support mental function under stress. Bacopa extract is thought to increase certain brain chemicals that are involved in thinking, learning, and memory. Use of plant tissue culture for micropropagation was initiated and he. Enhancing bacoside a production in shoot cultures was also investigated.
This paper reports a very simple, reproducible procedure for in vitro propagation of bacopa monnieri l. In vitro micropropagation of bacopa monnieri and detection of. Effect of cadmium, chromium, and lead on micropropagation. Bacopa monnieri is a perennial herb native to marshy areas of india, asia, and south america, that has been used for centuries in traditional ayurvedic medicine to prevent disease, promote healthy longevity, and strengthen the mind1 extensive research has been able to confirm that bacopa may be an effective anxiety reducer. The micro propagation technique has been a miraculous tool. Plants free fulltext in vitro propagation, phytochemical and. Bacopa monnieri has been shown to boost memory and cognition, improve mood, and reduce stress. Use of plant tissue culture for micropropagation was initiated and he found commercially viable approach for orchid propagation. In vitro culture of plants has gained importance during recent years. In vitro culture of highly valuable medicinal plant bacopa monnieri.
Micropropagation, in vitro flowering and cytological studies. Bacopa monnieri is a vegetatively propagated medicinal plant, which is endangered so it is necessary to develop micropropagation protocols to preserve the germplasm and distribute it to new cultivars during cultivation in new areas. Pdf an efficient micropropagation protocol of bacopa. Plantaginaceae and antimicrobial activity of in vitro and ex vitro raised plants. Bacopa monnieri has been used as a reputed drug in the indian traditional ayurvedic system for centuries.
Plants provide the predominant ingredients of medicines in most medical traditions. It has been revealed that micropropagation of bacopa monnieri could be carried out using axillary buds and internodal segments for producing true to type plants. In this study, the effect of cd, cr, and pb on the. Originally from the wetlands of india, and other southeastern asian countries. In vitro micropropagation of bacopa monnieri and detection. Optimization of micropropagation protocol of bacopa monnieri was carried out.
Pennell through twostage culture of nodal segments and ex vitro acclimatization. Various environmental factors affecting plant tissue culture are temperature, hydrogen ion concentration and light 14. Authentic dried plant oof bacopa monnieri was used to prepare the mother tincture. Among these, cadmium cd, chromium cr and lead pb are the most common heavy metals in the environment and can cause serious toxic effects on both animals and plants. In vitro conservation of bacopa monnieri 188 the different categories of plants and different plant parts constituting herbal medicines respectively. Bacopa monnieri supplement health benefits, dosage, side. Micropropagation of bacopa monnieri and enhancing bacoside. Micropropagation, in vitro flowering and cytological.
It is assumed that bacosides cross the bbb via lipid. Apart from india, nepal, sri lanka, china, taiwan and vietnam, it is also found in florida and other southern states of usa. Abstracts of the 26th annual meeting of the plant tissue culture association of india and national symposium on biotechnology for a better future. Bacopa monnieri is a rare and promising pharmaceutical uses plant.
In the ayurvedic bacopa has been recognized for its brain enhancement personality. Bacopa monnieri, clinical isolates, micropropagation, multidrugresistant, rti bacteria, spermidine, uti bacteria, background in more than 80% of developed countries, plants have been traditionally used as remedy because they are the actual good source of different pharmacologically active compounds 1. After collection of plants they were maintained in pots in the greenhouse of our institute. Effect of cadmium, chromium, and lead on micropropagation and. It is being used as a memory vitalizer in the indigenous systems of medicine. It can also decrease blood pressure both systolic and diastolic. Pase mp1, kean j, sarris j, neale c, scholey ab, stough c. Micropropagation of bacopa monnieri through protoplast author. The standard dose for bacopa monnieri is 300mg, assuming that the total bacoside content the active compound is 55% of the extract, by weight. Wettst a medicinally priced herb jaspreet kaur, kalpana nautiyal and manu pant department of biotechnology, graphic era university, dehradun, india corresponding author email. Bacopa monnieri, medicinal plant, two stage culture, ex vitro rooting. Pdf cost effective protocol for micropropagation of.
Nov 24, 2007 the regenerated plants resemble the mother plants in the general habitat without any morphological variation. Hardening of tissue culture desired plantlets are the most crucial step in the entire micropropagation process. Cost effective in vitro clonal propagation of bacopa monnieri l. Materials and methods source of explants plants of bacopa monnieri were obtained from sanjeevani plant nursery, bhopal m. In micropropagation of bacopa monnieri, a protocol for the sterilization, initiation and multiplication were achieved. A protocol has been developed for micropropagation of bacopa monnieri l. Bm demonstrates antioxidant, 5 hepatoprotective, 6 and neuroprotective 7 activity. After excision of the explants the most critical factor for successful establishment of cultures is the surface sterilization of the explants since contaminants usually reduce the. This shooting was achieved on low concentration of cytokinin with bap 1. Bacopa monnieri is an indian aquatic herb that has been used in traditional ayurvedic medicine for centuries for memory decline, pain, fevers, inflammation, and epilepsy. Tissue culture of endangered brahmi bacopa monnieri linn. Research article propagation of bacopa monnieri brahmi. To achieve the ideal 1020% of bacoside content requires a dose of 7501,500mg of the leaf or powder. In this article we dive into the history of bacopa, what are the benefits of bacopa when it comes to brain health, the side effects of bacopa, standard uses, dosages, and where to find the best sources of bacopa.
A costeffective and efficient protocol has been described in the present work for large scale and rapid in vitro propagation of a valuable medicinal herb bacopa monnieri l. Research suggests that it is also a neural protectant, safe. Bacopa monnieri, medicinal plant, twostage culture, ex vitro rooting. Bacopa monnieri, also known as brahmi, water hyssop or moneywort, has been used for centuries in ayurveda traditional medicine and has long been thought of as a wonder herb. To study the effect of vermicompost vc extracts on micropropagation of bacopa monnieri linn pennell. May 31, 2010 clonal propagation through tissue culture popularly called micropropagation. The species bacopa monnieri is a perennial, creeping, aquatic herb originally from the wetlands of southern india, australia, and south america. In vitro studies of bacopa monnierian important medicinal. Pdf in vitro micropropagation of brahmi bacopa monniera. International journal of recent biotechnology, 1, 2733.
Bacopa monnieri micropropagation acclimatization system preservation synthetic seeds rapd issr hplc gsh sod1 ros lung carcinoma introduction known as brahmi in india. Genus bacopa comprises of 146 species of aquatic herbs distributed throughout the warmer regions of the world. Tukeys studentized range hsd test has clearly indicated that development of leaves from nodes was significantly higher in humin alone without any supplements. Abstracts of the 26th annual meeting of the plant tissue culture association of india and national symposium on. Bacopa is a genus of as many as 100 aquatic plant species that are found throughout tropical and subtropical regions of the world. Bacopa, also known as water hyssop, is often referred to as brahmi. Residue obtained after acidbase treatment of vermicompost was used as plant tissue culture media for the micropropagation of bacopa monnieri. An efficient protocol for micropropagation of the rare medicinal herb bacopa monnieri l. Bacopa monnieri brahmi is a perennial, creeping, aquatic herb. Nodal segments containing axillary buds were surface sterilized with 0.
Pdf micropropagation of a medicinally important plant. The aim was to develop low cost and economical tissue culture media for. It is also reliable for improving memory formation. The world health organization who estimated that 80% of the population of developing countries relies on traditional medicines, mostly plant drugs, for their primary health care needs. Bacopa is cultivated in marshy area such as rice fields. Pdf improved micropropagation of bacopa monnieri l. Bacopa monnieri, commonly known as water hyssop, is an herb often used in ayurveda supplementing bacopa monnieri has been shown to improve cognition, by means of reducing anxiety. Bacopa monnieri activity, anticonvulsive action and antioxidant. Nowadays the multidrugresistant mdr bacterial pathogens are a major concern of the medical science. Pennell through twostage culture of nodal segments and ex vitro acclimatization article pdf available january 2015 with 519 reads.